The research work was conducted at the Research Laboratory of Biotechnology, Biochemistry, Biophysics Department of the Kuban State Agrarian University. The chief purpose of this study was to selection the most productive type of fungus of a genus of Trichoderma and optimal nutrient medium on basis of a waste of reprocessing of soy for acquire of protein enzyme feed supplement. For selection of a fungus of a genus Trichoderma used 3 types: Trichoderma viride, Trichoderma lignorum and Trichoderma harsianum. We used as a carrier for micromycete 3 types of nutrient media, which are based on soy okara and the additional sources of reprocessing of raw plant material – husk of a sunflower, husk of wheat (bran) and husk of rice. As indicators characterizing the efficiency of use micromycetes and substratum determined cellulolytic activity, protein, lignin, cellulose and reducing sugars content in the result mixture. The results demonstrated the benefits of using the nutrient medium based on soy okara forms and husk of a sunflower fungus to enhance Trichoderma lignorum production.