20-document - book paper5-communication - bubble chat talk conversation line107-document-folder tick done13-editorial - pencil write

Modeling of the cytokine environment, providing the optimal immune response of antitumor vaccines based on dendritic cells

All articles

Abstract

The inclusion of interferons and ligands TLR in the maturation cocktail provides not only a mature phenotype of DC, but also the prevalence of costimulatory molecules over coinhibitory, as well as high secretion of IL-12p70, but not IL-10, in response to CD40L stimulation; active directed migration in response to CCR7 ligands, and proliferation of a significant number of CTL in vitro. In contrast to standard DC vaccines, which are based on the use of either insufficiently mature DC (highly productive for IL-12, but with low migration and stimulatory activity) or mature DC (with high migration and stimulatory activity, but with reduced production of IL-12), the protocol developed in this study makes it possible to obtain a culture of αDC1, in which cells simultaneously combine all the necessary properties, even often contradictory properties of antitumor vaccines.